Identifying mRNA Sequence Elements for Target Recognition by Human Argonaute Proteins

ABSTRACT: It is commonly known that mammalian microRNAs guide the RNA-induced silencing complex (RISC) to target mRNAs through the seed-pairing rule. However, recent experiments by co-immunoprecipitating the argonaute proteins (AGOs), the central catalytic component of RISC, have consistently revealed extensive AGO-associated mRNAs lacking seed complementarity with microRNAs. We herein test the hypothesis that AGO has its own binding preference within target mRNAs, independent of guide microRNAs. By systematically analyzing the data from in vivo cross-linking experiments with human AGOs, we have identified a structurally accessible and evolutionarily conserved region (~10 nucleotides) that alone can accurately predict AGO-mRNA associations, independent of the presence of microRNA binding sites. With

ORGANISM(S): Homo sapiens

SUBMITTER: Zhaolei Zhang 

PROVIDER: E-GEOD-55122 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress