Identification and validation of antisense RNAs in Escherichia coli

ABSTRACT: In this study transcriptional start sites (TSS) of E. coli were determined for several growth conditions To detect the complement of transcripts expressed from E. coli, we collected two independent biological replicates (B1 and B2 samples) from MG1655 wild type strain grown to exponential (OD 600 ~0.4) or stationary phase (OD 600 ~2.0) in LB medium (samples LB 0.4 and LB 2.0, respectively) as well as grown to exponential phase (OD 600 ~0.4) in M63 minimal glucose medium (sample M63 0.4). For all six samples, total RNA was extracted and subjected to differential RNA-seq (dRNA-seq) library preparation for primary transcriptome analysis as described previously (Sharma et al., 2010). Specifically, prior to cDNA library construction half of each RNA sample was treated with 5M-bM-^@M-^Y terminat

ORGANISM(S): Escherichia coli str. K-12 substr. MG1655

SUBMITTER: Konrad FM-CM-6rstner 

PROVIDER: E-GEOD-55199 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress