Widespread Use of Non-Productive Alternative Splice Sites in Saccharomyces cerevisiae

ABSTRACT: Using RNA-Seq analysis of nonsense-mediated mRNA decay (NMD) mutant strains, we show that many Saccharomyces cerevisiae intron-containing genes exhibit usage of alternative splice sites, but most transcripts generated by splicing from these sites are non-functional because they introduce premature termination codons leading to transcript degradation by NMD. Analysis of splicing mutants combined with NMD inactivation revealed the role of specific splicing factors in governing the use of these alternative splice sites and identified novel functions for Prp17p in enhancing the use of branchpoint-proximal upstream 3M-bM-^@M-^Y splice sites and for Prp18p in suppressing the usage of a non-canonical AUG 3M-bM-^@M-^Y-splice site. The use of non-productive alternative splice sites can limit the ex

ORGANISM(S): Saccharomyces cerevisiae BY4741

SUBMITTER: Stephen Douglass 

PROVIDER: E-GEOD-55213 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress