Global impact of RNA polymerase II elongation inhibition on alternative splicing regulation (expression)

ABSTRACT: The rate of RNA polymerase II (pol II) elongation can influence splice site selection in nascent transcripts, yet the extent and physiological relevance of this kinetic coupling between transcription and alternative splicing is not well understood. We performed experiments to perturb pol II elongation and then globally compared alternative splicing patterns with genome-wide pol II occupancy. RNA binding and RNA processing functions were significantly enriched among the genes with pol II elongation inhibition-dependent changes in alternative splicing. Under conditions that interfere with pol II elongation, including cell stress, increased pol II occupancy was detected in the intronic regions flanking the alternative exons in these genes, and these exons generally became more included. A dis

ORGANISM(S): Homo sapiens

SUBMITTER: Qun Pan 

PROVIDER: E-GEOD-25287 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress