H3K27 modifications define segmental regulatory domains in the Drosophila bithorax complex.

ABSTRACT: The bithorax complex (BX-C) in the fruit fly, Drosophila melanogaster, is a cluster of homeotic genes that determines the identities the body segments. Expression of these genes is governed by cis-regulatory domains, one for each parasegment, which are arranged on the chromosome in the order of the parasegments they affect. Stable repression of these domains depends on the functions of the Polycomb Group, including its ability to methylate lysine 27 of histone H3. To learn whether PcG proteins generate parasegment-specific chromatin signatures, we have used transgenes to mark and isolate nuclei from single parasegments. These nuclei were profiled for histone modifications and chromatin proteins. The H3K27me3 profiles across the BX-C in successive parasegments show a striking “stairste

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Ruslan Sadreyev 

PROVIDER: E-GEOD-55257 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress