Systematic identification of regulatory elements in conserved 3’-untranslated regions of human transcripts

ABSTRACT: We present a combined experimental/computational technology to reveal a catalogue of functional regulatory elements embedded in 3’UTRs of human transcripts. We used a bidirectional reporter system coupled with flow cytometry and high-throughput sequencing to measure the effect of short, non-coding vertebrate-conserved RNA sequences on transcript stability and translation. Information-theoretic motif analysis of the resulting sequence-to-gene-expression mapping revealed linear and structural RNA cis-regulatory elements that positively and negatively modulate the post-transcriptional fates of human transcripts. We explored the functional role of 16332 short human 3'UTR sequences (C3U Library) evolutionarily conserved with P. troglodytes, M. musculus and G. gallus. For sorting the C3U library

ORGANISM(S): Homo sapiens

SUBMITTER: Panos Oikonomou 

PROVIDER: E-GEOD-55396 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress