High-resolution mapping reveals a conserved, widespread, dynamic meiotically regulated mRNA methylation program [Hs]

ABSTRACT: N6-methyladenosine (m6A) is a common modification of mRNA, with potential roles in fine-tuning the RNA life cycle, but little is known about the pathways regulating this process and its physiological role. Here, we used mass-spectrometry to identify a dense network of proteins physically interacting with METTL3, a core component of the methyltransferase complex, and show that two of them, WTAP and KIAA1429, are required for methylation. Combining high resolution m6A-Seq with knockdown of WTAP allowed us to define accurate maps, at near single-nucleotide resolution, of sites of mRNA methylation across four dynamic programs in human and mouse, including development, differentiation, reprogramming and immune response. Internal WTAP-dependent methylation sites were largely static across the d

ORGANISM(S): Homo sapiens

SUBMITTER: Schraga Schwartz 

PROVIDER: E-GEOD-55572 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress