Alternative Splicing of MBD2 Supports Self-Renewal in Human Pluripotent Stem Cells [HJAY]

ABSTRACT: Alternative RNA splicing (AS) regulates proteome diversity, including isoform-specific expression of several pluripotency genes. Here, we integrated global gene expression and proteomic analyses and identified a molecular signature suggesting a central role for AS in maintaining human pluripotent stem cell (hPSC) self-renewal. We demonstrate the splicing factor SFRS2 is an OCT4 target gene required for pluripotency. SFRS2 regulates AS of the methyl-CpG-binding protein MBD2, whose isoforms play opposing roles in maintenance of, and reprogramming to, pluripotency. While both MDB2a and MBD2c are enriched at the OCT4 and NANOG promoters, MBD2a preferentially interacts with repressive NuRD chromatin remodeling factors and promotes hPSC differentiation, whereas overexpression of MBD2c enhances r

ORGANISM(S): Homo sapiens

SUBMITTER: Hu Li 

PROVIDER: E-GEOD-55678 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress