Transcriptional profiling of an HtrA proteases knock-out compared to wild type
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ABSTRACT: Transcriptional profiling of an HtrA proteases knock-out compared to wild type on two different time points; logarithmic growth phase and stationary growth phase 3 Biological replicates per time point, two arrays
Project description:Transcriptional profiling of an HtrA proteases knock-out compared to wild type on two different time points; logarithmic growth phase and stationary growth phase
Project description:Transcriptional profiling of the wild-type and its htrA mutant. Identification of genes that are affected by the htrA mutation in P. gingivalis Keywords: Genetic modification Two-condition experiment, W83 vs. htrA mutant late-log growth phase. Biological replicates: 4 control, 4 mutant, independently grown. One replicate per array.
Project description:Trancriptional profiling of GE2-14 ?aguR mutant strain in which the aguR gene of the agmatine deiminase cluster has been deleted. We compared the expression profiling of GE2-14 cells with the expression profiling of GE2-14 ?aguR mutant cells. The goal was to determine the effect of the aguR deletion on the transcriptional profiling of GE2-14 Two strains GE2-14 (reference) vs. GE2-14 ?aguR (test) cells in same conditions. Three biological replicates, two technical replicates and one dye swap.
Project description:High temperature requirement A (HtrA) is a serine protease secreted by the group-I carcinogen Helicobacter pylori (H. pylori). The human cell adhesion protein and tumor suppressor E-cadherin (hCdh1) expressed on the surface of gastric epithelial cells was identified as the first HtrA substrate. HtrA-mediated hCdh1 cleavage and subsequent disruption of intercellular adhesion are considered as important steps in H. pylori pathogenesis. In this study, we performed a proteomic profiling of H. pylori HtrA (HpHtrA) to decipher the complex mechanism how H. pylori can interfere with epithelial barrier integrity.
Project description:In this work the effect of the hormone progesterone in the ability of C. albicans SC5314 to form biofilms was investigated as well as the transcriptomic response of the yeast to this compound. The analysis was performed always comparing the genomic expression of the biofilm cells cultivated in the presence and absence of progesterone with the transcriptome of planktonic cells (in mid-exponential phase) which was used to reference the analysis.
Project description:Microarray analysis revealed differential gene expression patterns of HT1080 cells treated with various chemical compounds alone and in combination (trabectedin, doxorubicin, mafosfamide, TRAIL, taurolidine) Microarray analysis of HT1080 cells treated with various chemical compounds alone and in combination (trabectedin, doxorubicin, mafosfamide, TRAIL, taurolidine)
Project description:Transcriptional profiling of the wild-type and its htrA mutant. Identification of genes that are affected by the htrA mutation in P. gingivalis Keywords: Genetic modification
Project description:The aim was to study the transcriptional profiling of the tdc cluster delection mutant E. faecalis V583 Î?tdc (non-tyramine producer) compared to the wild type strain E. faecalis V583 (tyramine producer). We compared the expression profile of the strains grown in M17 medium with glucose as carbon source and suplemented with tyrosine. E. faecalis V583 Î?tdc cells (test) compared with E. faecalis V583 cells (reference). Both strains grown in GM17 medium suplemented 15 mM tyrosine.
Project description:The goal was to determine the effect of agmatine on the trancriptional profile of L. lactis CECT 8666 strain. For that we compared the expression profile of L. lactis CECT 8666 cells grown in culture medium supplemented with 20 mM agmatine with the expression profile of L. lactis CECT 8666 cells grown in culture medium without agmatine. L. lactis CECT 8666 cells grown in GalM17 medium (reference) compared to L. lactis CECT 8666 cells grown in GalM17 medium supplemented with 20 mM agmatine (test).
Project description:Sortase-assembled pili contribute to virulence in many Gram-positive bacteria. In Enterococcus faecalis, the endocarditis and biofilm-associated pilus (Ebp) is polymerized on the membrane by sortase C (SrtC) and attached to the cell wall by sortase A (SrtA). In the absence of SrtA, polymerized pili remain anchored to the membrane (i.e. off-pathway). Here we show that the high temperature requirement A (HtrA) bifunctional chaperone/protease of E. faecalis is a quality control system that clears aberrant off-pathway pili from the cell membrane. In the absence of HtrA and SrtA, accumulation of membrane-bound pili leads to cell envelope stress and partially induces the regulon of the ceftriaxone resistance-associated CroRS two-component system, which in turn causes hyper-piliation and cell morphology alterations. Inactivation of croR in the ∆srtA∆htrA background partially restores the observed defects of the ∆srtA∆htrA strain, supporting a role for CroRS in the response to membrane perturbations. Moreover, absence of SrtA and HtrA decreases basal tolerance of E. faecalis against cephalosporins and daptomycin. The link between HtrA, pilus biogenesis and the CroRS two-component system provides new insights into the E. faecalis response to endogenous membrane perturbations.