Critical role of transient activation of human endogenous retroviruses during reprogramming toward pluripotency (Chip-Seq)

ABSTRACT: We recently showed that some human induced pluripotent stem cell (iPSC) clones were defective in neural differentiation and were marked with the activation of long term repeats (LTRs) of human endogenous retroviruses (HERVs). We herein demonstrated that these LTRs were transiently overexpressed during the generation of iPSCs and contributed to reprogramming. When the generation of iPSCs was completed, LTRs were re-suppressed to levels similar to those in human ES cells. However, differentiation-defective iPSC clones maintained high LTR expression levels, which indicated that these clones failed to complete reprogramming. lincRNA-RoR, a long intergenic non-coding RNA (lincRNA) that was previously shown to support the induction and maintenance of pluripotency, was detected among the LTR-driv

ORGANISM(S): Homo sapiens

SUBMITTER: Akira Watanabe 

PROVIDER: E-GEOD-56567 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress