Functional splicing network reveals extensive regulatory plasticity of the core Spliceosomal machinery

ABSTRACT: Pre-mRNA splicing relies on the still poorly understood dynamic interplay between more than 150 protein components of the Spliceosome, and the steps at which splicing can be regulated remain largely unknown. Here we systematically analyze the effect of knocking down the components of the splicing machinery on alternative splicing events relevant for cell proliferation and apoptosis and use this information to reconstruct a network of functional interactions. The network accurately captures well-established physical and functional associations and identifies new, revealing remarkable regulatory potential of core spliceosomal components, related to the order and duration of their recruitment during Spliceosome assembly. In contrast with standard models of regulation at early events

ORGANISM(S): Homo sapiens

SUBMITTER: Juan Valcarcel 

PROVIDER: E-GEOD-56605 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress