Argonaute-1 binds transcriptional enhancers and controls constitutive and alternative splicing in human cells

ABSTRACT: The roles of Argonaute proteins in cytoplasmic miRNA and RNAi pathways are well established. However, their implication in small RNA-mediated transcriptional gene silencing in the mammalian cell nucleus is less understood. We have recently shown that intronic siRNAs cause chromatin modifications that inhibit RNA polymerase II elongation and modulate alternative splicing in an Argonaute-1 (AGO1) dependent manner. Here we used chromatin immunoprecipitation followed by deep sequencing (ChIP-seq) to investigate the genome-wide distribution of AGO1 nuclear targets. Unexpectedly we found that about 80% of AGO1 clusters are associated with cell-type specific transcriptional enhancers, most of them (73%) overlapping active enhancers. This association seems to be mediated by long, rather than short

ORGANISM(S): Homo sapiens

SUBMITTER: Eneritz Agirre 

PROVIDER: E-GEOD-56826 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress