RAR/RXR binding dynamics distinguishes pluripotency from differentiation-associated cis-regulatory elements

ABSTRACT: In mouse embryonic cells, a retinoic acid (RA) stimulation triggers a massive change of gene expression leading the pluripotent, proliferating cells to a lineage-specific differentiation process. The retinoic acid receptor (RAR) plays a key role in this response by inhibiting pluripotency-maintaining genes and simultaneously activating some major actors of cell differentiation. To investigate the mechanism underlying this dual regulation, we performed joint RAR/RXR ChIP-seq and mRNA-seq time series during the first 48 hours of the RA-induced Primitive Endoderm differentiation process in F9 embryonic carcinoma cells. We detected significantly more RAR/RXR binding regions than previous studies and identified among them a handful of typical binding intensity patterns during differentiation. W

ORGANISM(S): Mus musculus

SUBMITTER: Philippe Veber 

PROVIDER: E-GEOD-56893 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress