Regulation of gene expression by Set-beta in rat retinal ganglion cells

ABSTRACT: The goal of this experiment was to investigate the molecular mechanism of how Set-beta regulates neurite growth. Set-betaM-bM-^@M-^Ys subcellular localization is regulated by posttranslational modifications. We found that Set-beta suppresses neurite growth of purified postnatal rat retinal ganglion cell (RGC) primary neurons when it is overexpressed in the nucleus, whereas recruiting Set-beta to cellular membrane by fusing myr-tag to its N-terminus promotes neurite growth. Here, we transfected purified by immunopanning postnatal rat RGC with wild-type Set-beta which localizes to the nucleus, myr-Set-beta which is recruited to cellular membranes, and mCherry control, and analyzed with microarrays Set-betaM-bM-^@M-^Ys subcellular localization-dependent effects on gene expression. We found th

ORGANISM(S): Rattus norvegicus

SUBMITTER: Ephraim Trakhtenberg 

PROVIDER: E-GEOD-56951 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress