Reprogramming of Endothelium Into Hematopoietic Progenitors by Defined Factors and Vascular Induction

ABSTRACT: Generation of abundant engraftable hematopoietic cells from autologous tissues promises new therapies for hematologic diseases. Differentiation of pluripotent stem cells into hematopoietic cells results in emergence of cells that have poor engraftment potential. To circumvent this hurdle, we have devised a vascular niche model to phenocopy the developmental microenvironment of hemogenic cells thereby enabling direct transcriptional reprogramming of human endothelial cells (ECs) into hematopoietic cells. In this approach, transduction of human umbilical vein ECs (HUVECs) or adult human dermal microvascular ECs (hDMECs) with transcription factors (TFs), FOSB, GFI1, RUNX1, and SPI1 (FGRS) and induction with a instructive vascular niche feeder layer in a xenobiotic- and serum-free microenviro

ORGANISM(S): Homo sapiens

SUBMITTER: Olivier Elemento 

PROVIDER: E-GEOD-57662 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress