Dataset Information

Secretome Comparison of Mesenchymal Stromal Cells and Immature Osteoblasts

ABSTRACT: Osteoblasts are a key component of the endosteal hematopoietic stem cell (HSC) niche and have long been recognized with strong hematopoietic supporting activity. Osteoblast conditioned media (OCM) enhances the growth of hematopoietic progenitors in culture and modulate their engraftment activity. We aimed to characterize the hematopoietic supporting activity of OCM by comparing the secretome of immature osteoblasts to that of their precursor, mesenchymal stromal cells (MSC). Over 300 secreted proteins were quantified by mass spectroscopy in media conditioned with MSC or osteoblasts, with 47 being differentially expressed.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Osteoblast, Cell Culture, Mesenchymal Cell, Bone Marrow

SUBMITTER: Owen HOvey

LAB HEAD: Nicolas Pineault

PROVIDER: PXD017372 | Pride | 2020-07-17

REPOSITORIES: Pride

ACCESS DATA

Publications

Insights into the Hematopoietic Regulatory Activities of Osteoblast by Secretomics.

Hovey Owen O Pasha Roya R Lehmann Zoe Z Pineault Nicolas N

Proteomics 20200714

Osteoblasts are a key component of the endosteal hematopoietic stem cell (HSC) niche and are recognized with strong hematopoietic supporting activity. Similarly, mesenchymal stromal cells (MSC)-derived osteoblast (M-OST) conditioned media (OCM) enhances the growth of hematopoietic progenitors in culture and modulate their engraftment activity. We aimed to characterize the hematopoietic supporting activity of OCM by comparing the secretome of M-OST to that of their precursor. Over 300 proteins we ...[more]

PMID: 32666692

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Dataset Information

Secretome Comparison of Mesenchymal Stromal Cells and Immature Osteoblasts

Publications

Insights into the Hematopoietic Regulatory Activities of Osteoblast by Secretomics.

Similar Datasets

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