RBFox2 regulates a broad RNA program and is required for genome-wide targeting of polycomb complex

ABSTRACT: Increasing evidence suggests that diverse RNA binding proteins interact with regulatory RNAs to regulate transcription. RBFox2 is a ubiquitous RNA binding protein with prevalent expression in stem cells, heart, and brain. Here, we report a systematic dissection of the RBFox2-regulated RNA program in the mouse heart, demonstrating its essential function for organizing the contractile apparatus and excitation-contraction (EC) coupling by modulating alternative splicing and microRNA activity. In this analysis, we unexpectedly encounter a major functional intersection between RBFox2 and Polycomb Complex 2 (PRC2), revealing a central requirement for RBFox2 to mediate genome-wide targeting of the PRC2 complex and transcriptional repression via chromatin-associated nascent RNAs. These findings un

ORGANISM(S): Mus musculus

SUBMITTER: Xiang-Dong Fu 

PROVIDER: E-GEOD-57926 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress