A novel phosphorylation site at Ser130 adjacent to the pseudosubstrate domain regulates protein kinase C-δ activity

ABSTRACT: Protein kinase C over-expressed into rat neonatal myocytes were lysed in 8M Urea, 0.1% SDS and trypsin digested. Peptides were fractionated by SPE-SCX and enriched by TiO2. Phosphopeptides were analyzed by LC/MS on a Thermo LTQ Elite Orbitrap. Raw files were searched using Sorcerer-Sequest and post search analysis and processing was done with Scaffold and Scaffold PTM.

INSTRUMENT(S):

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Cell Culture, Fetal Cardiomyocyte

SUBMITTER: Ronald Holewinski  

LAB HEAD: Jennifer Van Eyk

PROVIDER: PXD002536 | Pride | 2018-10-24

REPOSITORIES: Pride