Proteomics

Dataset Information

0

Integrated proteomics unveils regulation of cardiac myocytes hypertrophic growth by a nuclear cAMP nanodomain under the control of PDE3A

ABSTRACT: Signalling by cAMP is organized in multiple distinct subcellular nanodomains regulated by cAMP-hydrolysing phosphodiesterase (PDEs). Cardiac b-adrenergic signalling has served as the prototypical system to elucidate cAMP compartmentalization. Here we employed an integrated interaction and phosphoproteomics approaches that take advantage of the unique role that individual PDEs play in the control of local cAMP to identify previously unrecognizedncAMP nanodomains associated with b-adrenegic stimulation. The characterization of one such domain demonstrated that PDE3A isoforms operate in a nuclear nanodomain that involves SMAD4 and HDAC-1 to inhibit cardiac myocyte hypertrophic growth.

INSTRUMENT(S):

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Heart, Regular Ventricular Cardiac Myocyte, Fetal Cardiomyocyte

DISEASE(S): Disease Free

SUBMITTER: Duangnapa Kovanich  

LAB HEAD: Manuela Zaccolo

PROVIDER: PXD033773 | Pride | 2023-03-10

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
ORE01_KS_160629_ex2_001.raw Raw
ORE01_KS_160629_ex3_003.raw Raw
ORE01_KS_160629_ex4_005.raw Raw
ORE01_KS_170117_ex1_TiO2_01.raw Raw
ORE01_KS_170117_ex2_TiO2_03.raw Raw
Items per page:
1 - 5 of 24
altmetric image

Publications

<h4>Background</h4>Signaling by cAMP is organized in multiple distinct subcellular nanodomains regulated by cAMP-hydrolyzing PDEs (phosphodiesterases). Cardiac β-adrenergic signaling has served as the prototypical system to elucidate cAMP compartmentalization. Although studies in cardiac myocytes have provided an understanding of the location and properties of a handful of cAMP subcellular compartments, an overall view of the cellular landscape of cAMP nanodomains is missing.<h4>Methods</h4>Here  ...[more]

Similar Datasets

Proteomics Phosphodiesterase 9A Controls Nitric-oxide Independent cGMP and Hypertrophic Heart Disease
2016-06-24 | PXD001585 | Pride
Proteomics Constitutive Protein Kinase G Activation Exacerbates Stress-induced Cardiomyopathy
2023-07-20 | PXD022600 | Pride
Proteomics PP2A-B56alpha is a key determinant of cardiac protein phosphorylation and functional responses to beta-adrenergic signalling
2025-07-16 | PXD058709 | Pride
Proteomics Analyses of PDE-regulated phosphoproteomes reveal unique and specific cAMP signaling modules in T cells
2017-05-27 | MSV000081115 | MassIVE
Transcriptomics Comparison of neonatal and adult rat ventricular cardiomyocytes
2015-01-01 | E-MTAB-2832 | biostudies-arrayexpress
Proteomics Natural human tRNAAla anticodon variants mistranslate the genetic code
2025-03-07 | PXD053463 | Pride
Proteomics Multiplicity of regulatory subunit conformations define structural ensemble of reset Protein Kinase A holoenzyme
2025-07-14 | PXD061315 | Pride
Proteomics A positive feedback loop mediates crosstalk between calcium, cyclic nucleotide and lipid signaling in Toxoplasma gondii.
2022-10-17 | PXD030057 | Pride
Proteomics The AMA1-RON complex drives Plasmodium sporozoite invasion in the mosquito and mammalian hosts
2022-08-12 | PXD031463 | Pride
Genomics Reciprocal regulation of cardiac chromatin by the chromatin structural proteins HMGB and CTCF
2016-06-15 | E-GEOD-80453 | biostudies-arrayexpress