β-catenin regulates primitive streak induction through collaborative interactions with SMAD2/3 and OCT4

ABSTRACT: Canonical Wnt and Nodal signaling are both required for induction of the primitive streak (PS), which guides organization of the early embryo. The Wnt effector β-catenin is thought to function in these early lineage specification decisions via transcriptional activation of Nodal signaling. Here, we demonstrate a broader role for β-catenin in PS formation by analyzing its genome-wide binding in a human embryonic stem cell model of PS induction. β-catenin occupies regulatory regions in numerous PS and neural crest genes, and direct interactions between β-catenin and the Nodal effectors SMAD2/3 are required at these regions for PS gene activation. Furthermore, OCT4 binding in proximity to these sites is likewise required for PS induction, suggesting a collaborative interaction between β-caten

ORGANISM(S): Homo sapiens

SUBMITTER: Karen Schachter 

PROVIDER: E-GEOD-58476 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress