Growth arrest of human thyroid cancer cells 8505C after inhibition of TTF-1 transcriptional activity

ABSTRACT: Background: thyroid transcription factor-1 (TTF-1) is known to play key roles in thyroid organogenesis, in thyroid cell proliferation and in the expression of genes involved in thyroid differentiated function. Objective: to understand why many human thyroid cancer cell lines keep producing TTF-1 despite the loss of differentiation. Methods: a chimeric protein acting as a functional antagonist of TTF-1 transcriptional activity was expressed conditionally in 8505C cells originating from an undifferentiated thyroid carcinoma. The consequences of the inhibition of TTF-1 activity in the cells expressing the antagonist on cell proliferation, mRNA and miRNA expression were analyzed. Results: we observed a growth arrest of 8505C thyroid cancer cells when the endogenous TTF-1 transcriptional ac

ORGANISM(S): Homo sapiens

SUBMITTER: Frederick Libert 

PROVIDER: E-GEOD-58587 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress