Transcriptome impact of acute deletion of Gata3 in murine pro-T cells

ABSTRACT: The purpose of the study was to determine what genes in DN2 pro-T cells are immediately regulated by the transcription factor GATA-3, either as activation targets or as repression targets. To do this, two pairs of Gata3-floxed and control pro-T cells were generated and analyzed by RNA-seq within the first day of deletion of the Gata3 gene. Pro-T cells were generated by differentiation in vitro on OP9-DL1 monolayers of fetal liver-derive precursors from wildtype or Gata3-floxed mice, and the Gata3 gene was acutely deleted by transduction with Cre retroviral vector. Within 20 hr after transduction, samples of acutely Gata3-deleted and control DN2 cells were sorted and RNA prepared for RNA-seq analysis. High-throughput sequencing of the samples was carried out. Experimental Gata3 delete

ORGANISM(S): Mus musculus

SUBMITTER: Ellen Rothenberg 

PROVIDER: E-GEOD-59215 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress