Global regulatory networks active in Pseudomonas syringae B728 during leaf colonization and in response to target environmental stresses: the GacS, SalA, RetS, AlgU, HrpL, RpoN, RpoS, AhlR and AefR regulons

ABSTRACT: The plant pathogen Pseudomonas syringae pv. syringae B728a grows and survives on leaf surfaces and in the leaf apoplast of its host, bean. Global transcriptome profiling was used to understand the contribution of distinct regulators to B728a fitness and pathogenicity. We performed a transcriptome analysis of B728a and nine regulator mutants recovered from the surface and interior of leaves and exposed to various environmental stresses in culture. These mutants were nonpolar deletion mutants lacking a single target regulator gene: ahlR, aefR, gacS, retS, salA, rpoS, algU, hrpL and rpoN. RNA was collected from cells of these strains that were exposed to five treatments in vitro, namely a basal medium, sodium chloride to confer an osmotic stress, hydrogen peroxide to confer an oxidative stre

ORGANISM(S): Pseudomonas syringae

SUBMITTER: Gwyn Beattie 

PROVIDER: E-GEOD-59273 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress