Genome wide DNA methylation of Epstein-Barr virus infected immortalized normal oral keratinocytes

ABSTRACT: The oral cavity is the persistent reservoir for EBV with lifelong infection of resident epithelial and B cells. Infection of these cell types results in distinct EBV gene expression patterns that are regulated by epigenetic modifications involving DNA methylation and chromatin structure. Such regulation of EBV gene expression relies on viral manipulation of the host epigenetic machinery that may inadvertently result in long-lasting, oncogenic host epigenetic reprogramming. To test this hypothesis in the context of EBV infection of epithelial cells, we established a transient infection model to identify the epigenetic consequences after EBV infection of immortalized normal oral keratinocytes and subsequent viral loss. NOK cells were single cell cloned (designated as cl1) and EBV infected by

ORGANISM(S): Homo sapiens

SUBMITTER: Rona Scott 

PROVIDER: E-GEOD-59842 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress