Genome-wide RNA tomography in the zebrafish embryo

ABSTRACT: Advancing our understanding of embryonic development is heavily dependent on identification of novel pathways or regulators. While genome-wide techniques such as RNA sequencing are ideally suited for discovering novel candidate genes, they are unable to yield spatially resolved information in embryos or tissues. Microscopy-based approaches, using for example in situ hybridization, can provide spatial information about gene expression, but are limited to analyzing one or a few genes at a time. Here, we present a method where we combine traditional histological techniques with low-input RNA sequencing and mathematical image reconstruction to generate a high-resolution genome-wide 3D atlas of gene expression in the zebrafish embryo at three developmental stages. We also demonstrate that our t

ORGANISM(S): Danio rerio

SUBMITTER: Jan Philipp Junker 

PROVIDER: E-GEOD-59873 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress