Effects of miR-125b expression on the transcriptome of B cell precursors

ABSTRACT: Here, we have screened a miRNA expression library in a pre-B cell expression system to identify miRNAs with a potential oncogenic activity in early lymphocytes. We show that miR-125b is sufficient to transform B cell precursors in vitro as defined by growth factor independence, and demonstrate that continuous expression of miR-125b is necessary to keep cells in the transformed cells. Mechanistically, we find that expression of miR-125b protects against apoptosis induced by growth factor withdrawal, and that miR-125 interferes with the differentiation of pre-B to immature B cells. In consequence, transformed cells express the pre-BCR on the cell surface, which appears to provide signals for ongoing proliferation and survival. Using microarray analysis, we identified several previously known

ORGANISM(S): Mus musculus

SUBMITTER: Sebastian Herzog 

PROVIDER: E-GEOD-59994 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress