Dataset Information

In-depth analysis of miRNA binding sites reveals the complex response of uterine epithelium to miR-26a-5p and miR-125b-5p during early pregnancy

ABSTRACT: Post-transcriptional regulation of gene expression by miRNAs likely makes significant contributions to mRNA abundance at the embryo-maternal interface. In this study, we investigated how miR-26a-5p and miR-125b-5p contribute to molecular changes occurring in the uterine luminal epithelium, which serves as the first site of signal exchange between the mother and developing embryo. To measure de novo protein synthesis after miRNA delivery to primary uterine luminal epithelial cells, we employed pulsed stable isotope labeling by amino acids (pSILAC). We found that both miRNAs alter the proteome of luminal epithelial cells, impacting numerous cellular functions, immune responses, as well as intracellular and second messenger signaling pathways. Additionally, we identified several features of miRNA-mRNA interactions that may influence the targeting efficiency of miR-26a-5p and miR-125b-5p. Overall, our study suggests a complex interaction of miR-26a-5p and miR-125b-5p with their respective targets. However, both appear to cooperatively function in modulating the cellular environment of the luminal epithelium, facilitating the morphological and molecular changes that occur during the intensive communication between the embryo and uterus at pregnancy.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Sus Scrofa Domesticus (domestic Pig)

TISSUE(S): Uterine Epithelium Development, Endometrium Epithelium, Uterine Epithelium, Uterine Endometrial Epithelium

DISEASE(S): Disease Free

SUBMITTER: Piotr Zabielski

LAB HEAD: Piotr Zabielski, PhD

PROVIDER: PXD056243 | Pride | 2025-05-07

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	125b_peptides_1_1_0.mzid.gz	Mzid
	26a_peptides_1_1_0.mzid.gz	Mzid
	50515991kaczm_sw3C_26a_10.mgf	Mgf
	50515991kaczm_sw3C_26a_10.raw	Raw
	50515991kaczm_sw3C_26a_11.mgf	Mgf

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In-Depth Analysis of miRNA Binding Sites Reveals the Complex Response of Uterine Epithelium to miR-26a-5p and miR-125b-5p During Early Pregnancy.

Myszczynski Kamil K Szuszkiewicz Joanna J Krawczynski Kamil K Sikora Małgorzata M Romaniewicz Marta M Guzewska Maria M MM Zabielski Piotr P Kaczmarek Monika M MM

Molecular & cellular proteomics : MCP 20241112 1

Posttranscriptional regulation of gene expression by miRNAs likely makes significant contributions to mRNA abundance at the embryo-maternal interface. In this study, we investigated how miR-26a-5p and miR-125b-5p contribute to molecular changes occurring in the uterine luminal epithelium, which serves as the first site of signal exchange between the mother and the developing embryo. To measure de novo protein synthesis after miRNA delivery to primary uterine luminal epithelial cells, we used pul ...[more]

PMID: 39536955

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