Expression data from wheat cultivar TAM 107 flag leaf
Ontology highlight
ABSTRACT:
ORGANISM(S): Triticum aestivum
SUBMITTER: zhenshan liu
PROVIDER: E-GEOD-60351 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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Project description:We used microarray to study the transcriptome response of wheat flag leaves to heat stress (40℃)
2015-08-13 | GSE60351 | GEO
Project description:Heat stress adversely affects global bread wheat (Triticum aestivum L.) productivity. This study aimed to uncover the mechanisms underlying heat tolerance in wheat flag leaves and spike tissues. We compared protein abundance in flag leaves and spikes among wheat genotypes with contrasting heat tolerance—two tolerant (RAJ3765 and HD2932 )and two susceptible (HD2329 and HD2733)—under short-term heat stress (32°C) at ear peep. We identified 31 and 60 changes in protein abundance within flag leaves and spike tissues, respectively.
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Project description:To test whether non-coding RNAs play roles in regulating response to powdery mildew infection and heat stress in wheat, by using Solexa high-throughput sequencing and computational analysis and experimental approach we cloned the small RNAs and identified 125 putative long npcRNAs from wheat leaves infected by preponderant physiological strain Erysiphe graminis f. sp. tritici (Egt) or by heat stress treatment. Among long non-coding RNAs, some were precursors of small RNAs such as microRNAs and siRNAs, two long npcRNAs were identified as signal recognition particle (SRP) 7S RNA variants, and three were characterized as U3 snoRNAs. Wheat long npcRNAs showed tissue dependent expression patterns and were responsive to powdery mildew infection and heat stress. Examination non-coding RNAs of 2 near isogenic lines 8866 (Susceptible) and Pm30 (Resistant) in response to powdery milew and two genotypes CK (insensitive) and TAM107 (insensitive) to heat. CK and TAM107 represent the same material in different treatments (no heat stress or 1hour after heat stress).
2011-02-15 | E-GEOD-27327 | biostudies-arrayexpress
Project description:The protein content determines the cell state. The variation in protein abundance is crucial when organisms are in the early stages of heat stress, but the reasons affecting their changes are largely unknown. We quantified 47,535 mRNAs and 3,742 proteins in filling grain of wheat under two thermal environments. The impact of mRNA abundance and sequence features which implicated in protein translation and degradation on protein expression was evaluated by regression analysis. Transcription, codon usage and amino acid frequency mainly drive the changes in protein expression under heat stress, and their combined contribution explains 58.2% and 66.4% of protein variation in 30 and 40 °C, respectively. Of which, transcription contributes more to the alteration in protein content under 40 °C (31%) than to 30 °C (6%). Codon usage plays a stable and powerful role in protein expression under heat stress, even surpassing transcription. What’s more, the usage of AAG is a key factor regulating rapid protein expression under heat stress.
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Project description:Purpose: To identify abiotic stress responsive and tissue specific miRNAs at genome wide level in wheat (Triticum aestivum) Results: Small RNA libraries were constructed from four tissues (root, shoot, mature leaf and spikelets) and three stress treatments of wheat seedlings (control, high temperature, salinity and water-deficit). A total of 59.5 million reads were obtained by high throughput sequencing of eight wheat libraries, of which 32.5 million reads were found to be unique. Using UEA sRNA workbench we identified 47 conserved miRNAs belonging to 20 families, 1030 candidate novel and 51 true novel miRNAs. Several of these miRNAs displayed tissue specific expression whereas few were found to be responsive to abiotic stress treatments. Target genes were predicted for miRNAs identified in this study and their grouping into functional categories revealed that the putative targets were involved in diverse biological processes. RLM-RACE of predicted targets of three conserved miRNAs (miR156, miR160 and miR164) confirmed their mRNA cleavage, thus indicating their regulation at post-transcriptional level by corresponding miRNAs. Expression profiling of confirmed target genes of these miRNAs was also performed. Conclusions: This is the first comprehensive study on profiling of miRNAs in a variety of tissues and in response to several abiotic stresses in wheat. Our findings provide valuable resource for better understanding on the role of miRNAs in stress tolerance as well as plant development. Additionally, this information could be utilized for designing wheat plants for enhanced abiotic stress tolerance and higher productivity. Total eight (three stress, one control and four tissue specific small RNA libraries were pepared and sequenced independently [wheat control (WC), wheat high temperature stressed (WHTS), wheat salinity stressed (WSS) and wheat drought stressed (WDS), wheat shoot(WSH), wheat leaf (WLF), wheat flower(WFL), wheat root(WRT)] on Illumina GAIIx
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Project description:Calcium-dependent protein kinase (CPK) family is involved in diverse functions including abiotic tolerance, however, biological functions of some CPK members have not been clarified. In our previous study, abundance of a wheat CPK protein (TaCPK34) was remarkably induced during both grain and leaf organs of wheat plants suffering from various abiotic and biotic stresses, inferring its function involved in abiotic and biotic tolerance. In present study, its function involved in abiotic tolerance was further verified. Using Agroinfiltration-mediated transient transformation, TaCPK34 protein localizes to the plasma membrane in N. benthamina leaves. Its transcripts were significantly increased during 20% PEG-induced water deficiency, and its barley stripe mosaic virus-induced silencing wheat plants exhibited more sensitive phenotype to natural drought stress, suggesting that it could play key role in response to water deficiency in wheat. Isobaric tagging for relative and absolute quantification (iTRAQ) proteomic method further revealed that, in leaves of BSMV-VIGS-induced TaCPK34 silencing wheat plants, 48 protein species exhibiting significantly altered abundance were identified during water stress condition. The identified protein species were related to diverse functions (e.g. stress and defense, carbohydrate metabolism, nucleotide metabolic, photosynthesis, transportation, protein metabolism, signal transduction, lipid and phosphate metabolism), suggesting its potential regulatory mechanism. Our results provided insights on molecular mechanism of TaCPK34 on abiotic tolerance in higher plants.
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Project description:Genome-wide gene expression profiles in the leaves of two wheat genotypes, namely, heat susceptible 'Chinese Spring' (CS) and heat tolerant 'TAM107' (TAM) using GeneChip Wheat Genome Array
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Project description:Despite their importance, there remains a paucity of large scale expression-based studies of reproductive development in the species belonging to the Triticeae. As a first step to address this deficiency, a gene expression atlas of triticale reproductive development was generated using the 55K Affymetrix GeneChip® Wheat Genome Array. The global transcriptional profiles of the anther/pollen, ovary and stigma were analyzed at concurrent developmental stages and co-regulated as well as preferentially expressed genes were identified. Data analysis revealed both novel and conserved regulatory factors underlying Triticeae floral development and function. Triticale reproductive tissues (anther, ovary, stigma) were collected at 4 successive stages using pollen development as a developmental reference: tetrad (TET), uninucleate microspore (UNM), bi-cellular pollen (BCP), and tri-cellular pollen (TCP). Mature pollen grains (MPG) were also collected. Three biological replicates were analyzed for each tissue using the 55K Affymetrix GeneChip® Wheat Genome Array.
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Project description:We used two wheat genotypes, the susceptible wheat cultivar ‘8866 ’(S) and its near isogenic line with single powdery mildew resistance gene ‘pm30’ (R), to investigate gene expression changes in response to powdery mildew infection by using Wheat Genome Array wheat young leveas of near isogenic lines before or 12 hours after powdery mildew infection were selected for RNA extraction and hybridization on Affymetrix microarrays.The leaf samples were harvested from three independent biological replicates, and the leaves without inoculation were regarded as control.
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