Calibrating ChIP-seq with nucleosomal internal standards to measure histone modification density genome-wide

ABSTRACT: Despite serving as a central experimental technique in epigenetics research, chromatin immunoprecipitation (ChIP) suffers from several serious drawbacks: it is a relative measurement untethered to any external scale that obviates fair comparison amongst experiments; it employs antibody reagents that have differing affinity and specificity for target epitopes, which are in turn variable in abundance; and it is frequently not reproducible. To address these problems, we developed internal standard calibrated ChIP (ICeChIP), a method of spiking a native chromatin sample with nucleosomes reconstituted from recombinant and semisynthetic histones on barcoded DNA prior to immunoprecipitation. ICeChIP measures local histone modification densities on a biologically meaningful scale, enabling unbiase

ORGANISM(S): Mus musculus

SUBMITTER: Alexander Ruthenburg 

PROVIDER: E-GEOD-60378 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress