Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

ChIP-sequencing of the H3K4me1/2 in crypts from mice intestinal epithelium with intestinal epithelium specific LSD1 knock out compared to wild type

ABSTRACT: To study the epigenetic regulation of intestinal epithelium we focus on the role of chromatin modulators. Lysine-specific histone demethylase 1a (KDM1A, LSD1) is one of the enzymes that can erase the H3K4me1/2 mark. To assess the role of LSD1 in intestinal epithelium we studied wild type (WT) (Villin-Cre -; Lsd1f/f) and intestinal-epithelial-specific knock-out (KO) (Villin-Cre+; Lsd1f/f) mice. We found that KO mice completely lack Paneth cells, and have altered stem cell characteristics compared to WT littermates. To assess genome-wide H3K4me1/2 levels in WT and KO small intestines, we sorted small intestinal crypt cells, fixed them, isolated chromatin, and performed ChIP using an H3K4me1 and an H3K4me2 antibody as described in the protocols.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Mus musculus

SUBMITTER: Madeleine Fosslie

PROVIDER: E-MTAB-7871 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

LSD1 represses a neonatal/reparative gene program in adult intestinal epithelium.

Zwiggelaar Rosalie T RT Lindholm Håvard T HT Fosslie Madeleine M Terndrup Pedersen Marianne M Ohta Yuki Y Díez-Sánchez Alberto A Martín-Alonso Mara M Ostrop Jenny J Matano Mami M Parmar Naveen N Kvaløy Emilie E Spanjers Roos R RR Nazmi Kamran K Rye Morten M Drabløs Finn F Arrowsmith Cheryl C Arne Dahl John J Jensen Kim B KB Sato Toshiro T Oudhoff Menno J MJ

Science advances 20200911 37

Intestinal epithelial homeostasis is maintained by adult intestinal stem cells, which, alongside Paneth cells, appear after birth in the neonatal period. We aimed to identify regulators of neonatal intestinal epithelial development by testing a small library of epigenetic modifier inhibitors in Paneth cell-skewed organoid cultures. We found that lysine-specific demethylase 1A (<i>Kdm1a/Lsd1</i>) is absolutely required for Paneth cell differentiation. <i>Lsd1</i>-deficient crypts, devoid of Panet ...[more]

PMID: 32917713

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Dataset Information

ChIP-sequencing of the H3K4me1/2 in crypts from mice intestinal epithelium with intestinal epithelium specific LSD1 knock out compared to wild type

Publications

LSD1 represses a neonatal/reparative gene program in adult intestinal epithelium.

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