Secretion stress caused by overexpression of heterologous α-amylase in Bacillus subtilis

ABSTRACT: Transcriptome analysis was used to investigate the global stress response of the Gram-positive bacterium Bacillus subtilis caused by overproduction of the well-secreted AmyQ α-amylase from Bacillus amyloliquefaciens. Analyses of the control and overproducing strains were carried out at the end of exponential growth and in stationary phase, when protein secretion from B. subtilis is optimal. Among the genes that showed increased expression were htrA and htrB, which are part of the CssRS regulon that responds to high-level protein secretion and heat stress. The analysis of the transcriptome profiles of a cssS mutant compared to the wild-type, under identical secretion stress conditions, revealed several genes with altered transcription in a CssRS-dependent manner, for example citM, ylxF, ylo

ORGANISM(S): Bacillus subtilis subsp. subtilis str. 168

SUBMITTER: Andrzej Lulko 

PROVIDER: E-GEOD-8014 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress