Streptococcus gordonii gene response to the binding of human salivary amylase.

ABSTRACT: Streptococcus gordonii, an important primary colonizer of dental plaque biofilm, specifically binds to salivary amylase via the surface-associated amylase-binding protein A (AbpA). We hypothesized that amylase binding to S. gordonii modulates expression of chromosomal genes, which could influence bacterial survival and persistence in the oral cavity. Gene expression profiling by microarray analysis was performed to detect differentially expressed genes in S. gordonii strain CH1 in response to the binding of purified human salivary amylase as compared to exposure to heat-denatured amylase. Selected genes found to be differentially expressed were validated by qRT-PCR. Five genes from the fatty acid synthesis (FAS) cluster were highly (10-35 fold) up-regulated in amylase treated S. gordonii C

ORGANISM(S): Streptococcus gordonii str. Challis

SUBMITTER: Anna Nikitkova 

PROVIDER: E-GEOD-31830 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress