Dynamically reorganized chromatin is the key for the reprogramming of somatic cells to pluripotent cells

ABSTRACT: We investigated genome-wide nucleosome coverage and histone methylation occupancies in somatic MEFs, intermediate pre-iPSCs and fully reprogrammed iPSCs. We found that nucleosome occupancies increased in promoter regions and decreased in intergenic regions in pre-iPSCs and then recover to an intermediate level in iPSCs. Nucleosomes in pre-iPSCs are much more phased than those in MEFs and iPSCs. Bivalent, active, repressive domains are cell type-specific and change dynamically during reprogramming. HCG and LCG promoters exhibit distinct nucleosome occupancy patterns and are dynamically marked by H3K4me3/H3K27me3 during reprogramming, correspondingly showing different gene activities. Surprisingly, Vitamin C promotes nucleosome reorganization from pre-iPSCs to iPSCs. CTCF binding sites chang

ORGANISM(S): Mus musculus

SUBMITTER: Xiaobai Zhang 

PROVIDER: E-GEOD-60627 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress