Gene expression of Ustilago maydis SG200, SG200M-bM-^HM-^Fhxt1 and the avirulent SG200M-bM-^HM-^Fhxt1 hxt1(R164K) on maize leaf surface

ABSTRACT: Hxt1 is a high affinity hexose transporter important for virulence of the phytopathogenic basidiomycete Ustilago maydis on its host plant maize. Hxt1 shows the highest similarities to the glucose sensors Rgt2 and Snf3 from S. cereviciae (42% and 39% identity on amino acid level, respectively). In these sensors, the substitution of a highly conserved arginine to lysine leads to a constitutive active signal, resulting in expression of several glucose induced genes. Introduction of an analogous mutation in Hxt1 leads to loss of transport activity in the resulting Hxt1(R164K) protein. Expression of Hxt1(R164K) in hxt1 deletion strains results in a completely avirulent phenotype. Pathogenic developement of M-bM-^HM-^Fhxt1 hxt1(R164K) strains is blocked immediately after plant penetration. Expr

ORGANISM(S): Ustilago maydis

SUBMITTER: JM-CM-6rg KM-CM-$mper 

PROVIDER: E-GEOD-61069 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress