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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of brain and gut neurospheres of wild type and Bmi-1 knockout mice to reveal that Bmi-1 dependence distinguishes neural stem cell self-renewal from progenitor proliferation.

ABSTRACT: The cRNA derived from Brain and Gut neurospheres of wild type and BMI-1 knockout mice were hybridized to Affymetrix mouse ver 2 Chips A, B and C. In case of brain neurospheres, the cRNA were generated by one round RNA amplification (conventional method). In contrast, the cRNA of Gut neurospheres were generated by two round RNA amplifications.

ORGANISM(S): Mus musculus

SUBMITTER: Toshihide Iwashita

PROVIDER: E-GEOD-611 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Bmi-1 dependence distinguishes neural stem cell self-renewal from progenitor proliferation.

Molofsky Anna V AV Pardal Ricardo R Iwashita Toshihide T Park In-Kyung IK Clarke Michael F MF Morrison Sean J SJ

Nature 20031022 6961

Stem cells persist throughout life by self-renewing in numerous tissues including the central and peripheral nervous systems. This raises the issue of whether there is a conserved mechanism to effect self-renewing divisions. Deficiency in the polycomb family transcriptional repressor Bmi-1 leads to progressive postnatal growth retardation and neurological defects. Here we show that Bmi-1 is required for the self-renewal of stem cells in the peripheral and central nervous systems but not for thei ...[more]

PMID: 14574365

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