The catalytically inactive Domains Rearranged Methyltransferase3 controls DNA methylation and regulates RNA polymerase V transcript abundance in Arabidopsis

ABSTRACT: DNA methylation is a mechanism of epigenetic gene regulation and genome defense conserved in many eukaryotic organisms. In Arabidopsis, the DNA methyltransferase DRM2 controls RNA-directed DNA methylation in a pathway that also involves the plant specific RNA Polymerase V (Pol V). The Arabidopsis genome also encodes an evolutionarily conserved but catalytically inactive DNA methyltransferase DRM3. Here, we show that DRM3 has moderate effects on global RNA-directed DNA methylation and small RNA abundance throughout the genome, and DRM3 protein physically interacts with Pol V. In drm3 mutants, we observe a lower level of Pol V-dependent transcripts, even though Pol V chromatin occupancy is increased at many sites in the genome. These findings suggest that DRM3 acts to promote Pol V transcri

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Christopher Hale 

PROVIDER: E-GEOD-61192 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress