The general mode of translation inhibition by macrolide antibiotics

ABSTRACT: Macrolides are clinically important antibiotics thought to inhibit bacterial growth by impeding the passage of newly synthesized polypeptides through the nascent peptide exit tunnel of the bacterial ribosome. Recent data challenged this view by showing that macrolide antibiotics can differentially affect synthesis of individual proteins. In order to understand the general mechanism of macrolide action, we used genome-wide ribosome profiling and analyzed the redistribution of ribosomes translating highly expressed genes in bacterial cells treated with high concentrations of macrolide antibiotics. The metagene analysis indicated that inhibition of early rounds of translation, which would be characteristic of the conventional view of macrolide action, occurs only at a limited number of genes.

ORGANISM(S): Escherichia coli BW25113

SUBMITTER: Krishna Kannan 

PROVIDER: E-GEOD-61619 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress