Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

RNA-seq analysis of Mst1/2 deleted bronchiolar epithelial cells from adult mouse lungs

ABSTRACT: Mst1 and Mst2 were conditionally deleted from non-ciliated bronchiolar epithelial cells in the mature lung. Bronchiolar epithelial cells from control and Mst1/2 deleted mice were isolated by cell sorting and used for RNA-seq analysis. Scgb1a1-rtTA/tetO-Cre/Mst1;2-flx/flx (Mst1/2 D/D) mice were generated to conditionally delete Mst1 and Mst2 from non-ciliated, secretory bronchiolar epithelial cells. Adult mice were maintained on doxycycline food for 16 days to induce deletion of Mst1/2. Lin-/CD326+/CD24-intermediate cells were isolated by fluorescence cell sorting to enrich for the targeted airway epithelial cells. mRNA isolated from Lin-/CD326+/CD24-intermediate cells from control and Mst1/2 D/D mice was pooled and analyzed by RNA-seq to identify transcriptional changes following deletion of Mst1 and Mst2 from mature lung bronchiolar epithelial cells.

ORGANISM(S): Mus musculus

SUBMITTER: Alexander Lange 

PROVIDER: E-GEOD-61627 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Json Xml
altmetric image

Publications

Hippo/Yap signaling controls epithelial progenitor cell proliferation and differentiation in the embryonic and adult lung.

Lange Alexander W AW   Sridharan Anusha A   Xu Yan Y   Stripp Barry R BR   Perl Anne-Karina AK   Whitsett Jeffrey A JA  

Journal of molecular cell biology 20141205 1

The Hippo/Yap pathway is a well-conserved signaling cascade that regulates cell proliferation and differentiation to control organ size and stem/progenitor cell behavior. Following airway injury, Yap was dynamically regulated in regenerating airway epithelial cells. To determine the role of Hippo signaling in the lung, the mammalian Hippo kinases, Mst1 and Mst2, were deleted in epithelial cells of the embryonic and mature mouse lung. Mst1/2 deletion in the fetal lung enhanced proliferation and i  ...[more]

Similar Datasets

Unknown RNA-seq analysis of Mst1/2 deleted bronchiolar epithelial cells from adult mouse lungs
2015-01-01 | GSE61627 | GEO
Transcriptomics Microarray of Mst1/2 deleted epithelial cells from E18.5 mouse lungs
2015-01-01 | E-GEOD-61582 | biostudies-arrayexpress
Unknown Microarray of Mst1/2 deleted epithelial cells from E18.5 mouse lungs
2015-01-01 | GSE61582 | GEO
Transcriptomics Expression data from Mst knockout embryoid bodies and wild type embryoid bodies
2014-01-02 | E-GEOD-50219 | biostudies-arrayexpress
Unknown Expression data from Mst knockout embryoid bodies and wild type embryoid bodies
2014-01-02 | GSE50219 | GEO
Transcriptomics Mst1/2-Yap in lung epithelial progenitor cells
2015-01-01 | E-GEOD-61628 | biostudies-arrayexpress
Unknown Hippo kinases Mst1 and Mst2 sense and amplify IL-2R−STAT5 signaling in Treg cells to establish stable regulatory activity
2018-11-08 | GSE120711 | GEO
Unknown C/EBPa Regulates Protease/anti-protease Balance and Mediates Bronchiolar Cell Recovery After Injury
2012-05-13 | GSE29285 | GEO
Transcriptomics C/EBPa Regulates Protease/anti-protease Balance and Mediates Bronchiolar Cell Recovery After Injury
2012-05-12 | E-GEOD-29285 | biostudies-arrayexpress
Unknown Hippo signaling coordinates cellular quiescence with terminal maturation in iNKT cell development and fate decisions [ClariomS]
2020-04-18 | GSE144776 | GEO