Correlating citric acid formation and manganese limitation in Aspergillus niger using transcriptome and proteome analysis

ABSTRACT: Aspergillus niger is well known for its capability to produce citrate in high amounts but the detailed metabolic response causing citrate production has not been fully elucidated. Manganese is known to have an important effect as its limitation is a requirement to obtain high-level citrate formation. To identify the translational regulation causing citric acid overflow metabolism, transcriptome and proteome data from cultivations in manganese limitation and manganese excess conditions were analyzed. In addition to four already described main responses, two novel events were identified. The first metabolic response was down regulation of phosphoenolpyruvate carboxykinase (PEPCK) during manganese limited conditions, which was confirmed by in vivo experiments. Down regulation of the first ste

ORGANISM(S): Aspergillus niger

SUBMITTER: Lars Poulsen 

PROVIDER: E-GEOD-61985 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress