Upstream Anti-sense Promoters Act as Local Enhancers of Mammalian Protein-coding Genes

ABSTRACT: Anti-sense transcription originating upstream of mammalian protein-coding genes is a well-documented phenomenon, but remarkably little is known about the function or regulation of these anti-sense promoters or the non-coding RNAs they generate. Here we define at nucleotide resolution the divergent transcription start sites (TSSs) near mouse mRNAs. We find that coupled sense and anti-sense TSSs form the boundaries of an evolutionarily conserved and nucleosome-depleted regulatory region with dramatically enriched transcription factor (TF) occupancy. Notably, as the distance between sense and anti-sense TSSs increases, so does the level of TF binding and signal-dependent gene activation. We further discover a cluster of anti-sense TSSs in macrophages with an enhancer-like chromatin signature.

ORGANISM(S): Mus musculus

SUBMITTER: Karen Adelman 

PROVIDER: E-GEOD-62151 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress