Comparison of murine whole gene expression on ligand immobilized surfaces

ABSTRACT: For the studies of cell migration in various micro-environments, self-assembled monolayer surfaces presenting adhesive ligand, RGD peptide, were used for the assay. Presentation of RGD ligands was based on the immobilization reaction with alkanethiols on a gold surface. There were differences in cell morphology and migration according to the ligand affinity (linear vs. cyclic RGD) and manner of ligand presentation (dynamic vs. non-dynamic). In dynamic surface experiment, cells initially grown on the fibronectin-coated patterns were exposed to either linear or cyclic RGD while in non-dynamic surface experiment cells interacted with the immobilized RGD from the beginning. Goal was to compare how the affinity and spatio-temporal exposure of ligand affect the overall transcriptional profiling.

ORGANISM(S): Mus musculus

SUBMITTER: Eun-ju Lee 

PROVIDER: E-GEOD-62304 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress