Defining the nociceptor transcriptome

ABSTRACT: Unbiased 'omics' techniques, such as next generation RNA-sequencing, can provide entirely novel insights into biological systems. However, cellular heterogeneity presents a significant barrier to analysis and interpretation of these datasets. The neurons of the dorsal root ganglia (DRG) are an important model for studies of neuronal injury, regeneration and pain. The majority of investigators utilize a dissociated preparation of whole ganglia when studying cellular and molecular function. We demonstrate that the standard methods for producing these preparations gives a 10%-neuronal mixture of cells, with the remainder of cells constituting satellite glia and other non-neuronal cell types. Using a novel application of magnetic purification, we consistently obtain over 95% pure, viable neuro

ORGANISM(S): Mus musculus

SUBMITTER: Megan Crow 

PROVIDER: E-GEOD-62424 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress