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Transcription profiling of mouse dorsal root ganglia after diphtheria-toxin deletion of the Nav1.8-expressing cells using the cre/loxP system

ABSTRACT: Comparison of transcript levels after diphtheria-toxin deletion of the Nav1.8-expressing cells using the cre/loxP system.
Pain requires input from nociceptors which are specialised peripheral sensory neurons in dorsal root ganglia (DRG). To explore the nociceptor-specific mRNA profiles, we used microarray analysis to examine the altered repertoire of genes expressed in DRG from mice depleted of Nav1.8-expressing neurons which are mainly nociceptors. To generate the animal, a Nav1.8 knock-in Cre-expressing mouse (Nav1.8-Cre) was used to excise a floxed stop upstream of globally expressed diphtheria toxin A-subunit gene (ROSA26-eGFP-DTA). By crossing heterozygous Cre mice with homozygous toxin-expressing floxed mice, experimental toxin-expressing (DTA) mice, in which DTA expressing nociceptors had been deleted, were generated. 6 DTA mice and 6 litter mate controls were used for microarray analysis. 3 Affymetrix Mouse Genome 430 2.0 Array chips were employed for analysis of the gene transcripts of each conditions.

ORGANISM(S): Mus musculus

SUBMITTER: Jing Zhao

PROVIDER: E-MEXP-1622 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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BENZOXAZINONE DERIVATIVES, THE PREPARATION AND USE THEREOF AS MEDICAMENTS

TORRENS-JOVER ANTONIO CASTRILLO-PEREZ JOSE AURELIO FRIGOLA-CONSTANSA JORDI

20030409

The invention relates to novel compounds of general formula (I), to their physiologically acceptable salts, to methods for the preparation thereof, to intermediate compounds for the obtention of said compounds, to the use of compounds of general formula (I) as medicaments in the prevention and treatment of central nervous system disorders and to the pharmaceutical compositions containing said compounds. ...[more]

PMID: WO03084939

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