Evaluation of DNA array sensitivity to detect viruses in clinical samples following propidium monoazide treatment

ABSTRACT: Pan-viral DNA array (PVDA) and high-throughput sequencing (HTS) are useful tools to identify novel virus of emerging diseases. However, both techniques have difficulties to identify viruses in clinical samples because of host genomic DNA (hgDNA) contamination. Both propidium monoazide (PMA) and ethidium bromide monoazide (EMA) have the capacity to bind free DNA but are cell membrane-impermeable and thus are unable to bind protected DNA and RNA such as viral genomic material. DNA modified by EMA or PMA is not amplifiable by polymerase. In order to assess the capacity of EMA or PMA to lower hgDNA, serum or lung tissue homogenates were spiked with porcine reproductive and respiratory virus (PRRSV) and were processed with different combination of treatment: with or without ultracentrifugation

ORGANISM(S): Sus scrofa

SUBMITTER: Christian Bellehumeur 

PROVIDER: E-GEOD-62910 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress