RNASeq of MARC-145 or MA-104 cells infected with porcine reproductive and respiratory syndrome virus (PRRSV)-1 or PRRSV-2, harvested at 3-12 hpi

ABSTRACT: Cell lines derived from Chlorocebus sabaeus kidney were infected with an isolate of PRRSV-1 or PRRSV-2 and RNASeq was performed (this entry) in parallel with ribosome profiling (see related accession numbers). These RNASeq datasets provide information on the transcriptome of PRRSV-infected cells and were used to detect novel viral transcripts and perform differential gene expression analysis on host transcripts. For the PRRSV-1 experiments, MA-104 cells were infected with an isolate based on the Porcilis vaccine strain (KJ127878.1 but with several accumulated mutations, see associated publication) and harvested at 8 hpi after pre-treatment with cycloheximide (CHX). For the PRRSV-2 experiments, MARC-145 cells were infected with SD95-21 PRRSV (KC469618.1), and a mutant thereof (KO2). One gro

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Chlorocebus sabaeus

SUBMITTER: Georgia Cook 

PROVIDER: E-MTAB-10623 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress