Ribosome profiling of MARC-145 or MA-104 cells infected with porcine reproductive and respiratory syndrome virus (PRRSV)-1 or PRRSV-2, harvested at 3-12 hpi

ABSTRACT: Cell lines derived from Chlorocebus sabaeus kidney were infected with an isolate of PRRSV-1 or PRRSV-2 and ribosome profiling was performed (this entry) in parallel with RNASeq (see related accession number). These datasets were used to analyse the viral and host translatome, frameshifting on the viral genome, and putative frameshift-related ribosome pausing events. For the PRRSV-1 experiments, MA-104 cells were infected with an isolate based on the Porcilis vaccine strain (KJ127878.1 but with several accumulated mutations, see associated publication) and harvested at 8 hpi after pre-treatment with cycloheximide (CHX). For the PRRSV-2 experiments, MARC-145 cells were infected with SD95-21 PRRSV (KC469618.1), and a mutant thereof (KO2). One group of samples was harvested at 9 hpi after pre-

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Chlorocebus sabaeus

SUBMITTER: Georgia Cook 

PROVIDER: E-MTAB-10621 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress