Efficient generation of stable chondrocytes from human iPSC through early induction of a pre-chondrogenic population

ABSTRACT: Regeneration of human cartilage is inherently inefficient; an abundant autologous source like human induced pluripotent stem cells (hiPSC) is therefore attractive for engineering cartilage. Here, we report a defined growth factor based protocol for differentiating hiPSC into articular-like chondrocytes within two weeks with a high efficiency. The hiPSC-derived chondrocytes (hiChondrocytes) are stable and comparable to adult articular chondrocytes in global gene expression, extracellular matrix production and in their ability to generate cartilage tissue in vitro and in immune-deficient mice. Molecular characterization identified an early Sox9lowCD44lowCD140low pre-chondrogenic mesodermal population during hiPSC differentiation that eventually generates a homogenous population of Sox9highCD

ORGANISM(S): Homo sapiens

SUBMITTER: Sarah Taylor 

PROVIDER: E-GEOD-62914 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress