RNA sequencing (RNA-SEQ) of EPAS1 knockdown by siRNA in endothelial cells

ABSTRACT: Purpose: By integrating DNA methylation and gene expression of COPD lung tissues, we identified EPAS1 as a key regulator whose downstream genes significantly overlapped with multiple genes sets associated with COPD disease severity. EPAS1 is distinct in comparison with other key regulators in terms of methylation profiles and downstream target genes. Genes predicted to be regulated by EPAS1 were enriched for biological processes including signaling, cell communications, and system developement. As EPAS1 downstream genes were significantly enriched for hypoxia responsive genes in endothelial cells, we tested EPAS1 function in human and mouse endothelial cells HUVEC and C166. EPAS1 knockdown by siRNA in endothelial cells impacted genes that significantly overlapped with EPAS1 downstream gene

ORGANISM(S): Homo sapiens

SUBMITTER: Jun Zhu 

PROVIDER: E-GEOD-62974 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress