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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Next Generation Sequencing Comparison of C57BL/6J and BC027072 -/- Eye Transcriptomes

ABSTRACT: We report the single base pair analysis of the ocular transcriptome from wild type and BC027072 knockout animals. Comparison was analyzed to understand gene expression changes in a mouse model for early onset retinal degeneration which phenocopies a human form of autosomal recessive retinitis pigmentosa Eye mRNA profiles were generated from 3 week-old C57BL/6J and BC027072 -/- in triplicate and sequenced using the Illumina HiSeq 2500

ORGANISM(S): Mus musculus

SUBMITTER: Brian Kevany

PROVIDER: E-GEOD-63810 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Animals deficient in C2Orf71, an autosomal recessive retinitis pigmentosa-associated locus, develop severe early-onset retinal degeneration.

Kevany Brian M BM Zhang Ning N Jastrzebska Beata B Palczewski Krzysztof K

Human molecular genetics 20150123 9

Genetic mapping was recently used to identify the underlying cause for a previously uncharacterized cohort of autosomal recessive retinitis pigmentosa cases. Genetic mapping of affected individuals resulted in the identification of an uncharacterized gene, C2Orf71, as the causative locus. However, initial homology searches failed to reveal similarities to any previously characterized protein or domain. To address this issue, we characterized the mouse homolog, BC027072. Immunohistochemistry with ...[more]

PMID: 25616964

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