Next Generation Sequencing Identifies Differentially Expressed Genes in Zebrafish Embryos and Larvae Following Benzo[a]pyrene Exposure

ABSTRACT: Purpose: This study aimed to identify differentially expressed genes and transcripts in zebrafish embryos and larvae following benzo[a]pyrene (BaP) exposure. Methods: Adult zebrafish (2 males × 4 females, N=6 replicate tanks for each treatment) were acclimated for 7 days in an 818 Low Temp Illuminated Incubator (Precision Scientific, Chennai, India) at 28.5°C. Next, adult fish were waterborne exposed to control or 50 μg/L (ppb) BaP for 7 days; ethanol was used as vehicle solvent, and final ethanol concentration was 0.1 mL/L (100 ppm) in all treatment groups. This dose of ethanol is not teratogenic to zebrafish. Water was changed and/or re-dosed daily. From day 7 to 11 of the parental exposure, eggs were collected, counted, and raised in normal conditions (control) or continuously exposed t

ORGANISM(S): Danio rerio

SUBMITTER: Xiefan Fang 

PROVIDER: E-GEOD-64198 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress